Part:BBa_K3056000:Design

Anti-THC antibody fragment (ScFv) linked to mNG at the C-terminal

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 691
  Illegal AgeI site found at 916
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

mNG was chosen as the fluorescent protein, as it is the brightest available fluorescent protein. mNG was linked to the C-terminal, rather than the N-terminal, to avoid blocking the antigen binding site of the antibody. OmpA recognition protein sequence was placed upstream of the ScFv coding sequence to direct the protein to the periplasm.

Source

The antibody fragment was produced by Dr. Brennan's "The Production of Recombinant single chain Antibody Fragments for the Detection of Illicit Drug Residues". The mNG sequence was obtained from Branchiostoma lanceolatum by Shaner et al. (2013).

References

1. Brennan, J. (2005) The production of recombinant single chain antibody fragments for the detection of illicit drug residues. doctoral thesis, Dublin City University, [online] http://doras.dcu.ie/17319/ (Accessed March 12, 2019)